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KMID : 0380219930260050460
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Journal of Biochemistry and Molecular Biology
1993 Volume.26 No. 5 p.460 ~ p.464
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Cell Cycle Dependent Expression of cdc2 During Rat Liver Regeneration
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Chung-Ho Hong
Hee Won Lee/Sung-Ki Lee/Kyung Hee Choi and Doe Sun Na
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Abstract
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The cdc2 gene encodes a 34 kDa protein (p34^(cdc2)) which is a protein kinase and plays a regulatory role in the cell cycle. To study the regulation of cdc2 in regenerating rat liver, we have cloned rat cdc2 cDNA from a rat liver cDNA library. The nucleotide sequence of the coding region, and the deduced amino acid sequence for 297 residues, show, respectively, 94.1% and 99.3% homologies to mouse cdc2. Expression of cdc2 during the first cell cycle of rat liver regeneration after partial hepatectomy was investigated. The level of mRNA was low until entry into S phase, increased to a maximum during S and G2 phase, decreased during M phase, and dropped to the background level upon exit from M phase. The pattern of p34^(cdc2) activity level change was similar to the that of mRNA.
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